Enzymes in Recombinant DNA technology

Which type of restriction endonuclease is used in recombinant DNA technology

The enzymes used to cut and join DNA are

Enzyme that removes a phosphate group from 5' ends of DNA

• Acid phosphatase

• Lambda exonuclease

• Alkaline phosphatase

• Terminal transferase

Enzyme that can add homopolymer tails to 3' ends of DNA

• Terminal transferase

• DNA polymerase

• Lambda exonuclease

• Polynucleotide kinase

Name a restriction Endonuclease (no space in between)

Type II Restriction Endonucleases recognise....

.,....... Sequence

Enzyme that can introduce a small gap of 2 to 3 nucleotides in DNA are

• S1 nuclease

• Restriction Endonuclease

• Lambda exonuclease

Lambda exonuclease cleaved DNA is an excellent substrate for the enzyme

• DNA polymerase

• Terminal transferase

• Polynucleotide kinase

• Alkaline phosphatase

Enzyme that forms a phosphodiester bond between 3'OH of one DNA strand and 5'P of another DNA strand

A Restriction Endonuclease (without spacing) that creates a 3' overhang