PRE-TEST: CONNECTIVE TISSUES AND AMYLOID

Connective tissues are divided into the following

groups

• Blood

• Bone

• Cartilage

• Loose or areolar

Intercellular substances may classified into main groups by their microscopic appearance:

• amorphous

• formed

• gel types

• fibrous types

Diagram shows reticular fiber pattern in normal liver by the silver impregnation method (1936). What is the possible staining?

• van Gieson

• Verhöeff’s

• Resorcin-fuchsin

• Gordon and Sweets

Diagram shows a section of skin demonstrating

collagen in the dermis. The staining involved is

• van Gieson

• Verhöeff’s

• Resorcin-fuchsin

• Masson trichrome

Choose correct statement(s) about collagenic fibers

• strongly birefringent under light microscope

• subdivided into type I, II and III

• most frequently encountered of all the fibrous types

• may occur as large bundles of fibers clumped together

Factors affecting trichrome staining include:

• pH

• heat

• tissue permeability

• dye molecular size

What will enhance trichrome intensity and brilliance in trichrome staining?

• Use of formaldehyde-fixed tissues

• Prolong fixation in formaldehyde

• Use of Bouin’s fixative

• Treatment of tissues with picric acid or mercuric chloride solutions

Choose the correct PTAH staining result

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This special staining demonstrate....

• reticular fibers

• collagen fibers

• muscle striation

• elastic fibers

This type of fibers could be demonstrated using special staining called....

• trichrome staining

• van Gieson staining

• Gordon and Sweets staining

• PTAH staining