EI8551-AI-UNIT 2-2.8- Quiz - High Pressure Liquid Chromatogr

Which of the following is not true about High pressure liquid chromatography (HPLC)?

• a) It requires high pressure for the separation of the specious

• b) There is no need to vaporise the samples

• c) It is performed in columns

• d) It has high sensitivity

High pressure liquid chromatography can be performed only in columns.

• a) True

• b) False

Which of the following is not an advantage of Syringe type pumps used in High pressure liquid chromatography?

• a) Independent of viscosity

• b) Pulse-less flow

• c) High pressure capability

• d) Unlimited solvent capacity

Which of the following is not true about solvent programming which is done in high performance liquid chromatography?

• a) It provides unequal bandwidths

• b) It provides fast overall separation

• c) It provides maximum resolution

• d) It provides maximum sensitivity

Which of the following pulse damper takes up some amount of the pulsation energy which is released to provide smooth pressure without pulsations?

• a) Flexible bellows or compressible gas passed through tee columns

• b) Flexible inert diaphragm

• c) Electronic pulse damper

• d) Electrical pulse damper

Which of the following is not a characteristic of the syringe pump used in high pressure liquid chromatography?

• a) Pressure capability is high

• b) Maintenance is frequent

• c) Limited reservoir capability

• d) Slight change of flow rate when extremely high pressure compresses the solvent

Syringe pumps used in High pressure liquid chromatography are most suitable for which of the following columns?

• a) Capillary columns

• b) Guard columns

• c) Short-fast columns

• d) Small bore columns

Gravity feed method for solvent delivery is not used with narrow bore columns packed with fine mesh particles

• a) True

• b) False

Which of the following cannot be done to reduce ripple in High pressure liquid chromatography?

• a) Using bellow

• b) Using restrictors

• c) Using long nylon tube between pump and column

• d) Avoiding the use of the solvent pump

Which of the following is not true about Hydraulic capacitance flow control system used in HPLC?

• a) It can be used only for liquids with low viscosity

• b) It is irrespective of solvent compressibility

• c) It maintains a constant flow

• d) It smoothens high pressure pump pulsations