Yeast expression systems

Why is a eukaryotic cloning vector required?

• a) Cloning ease

• b) Variety

• c) Availability

• d) Large scale production

Saccharomyces cerevisiae is an example of _________

• a) Fungi

• b) Yeast

• c) Bacteria

• d) Insect

Which selection system is used in a yeast plasmid recombinant?

• a) Antibiotic

• b) Lac

• c) Auxotrophic mutant

• d) cI gene

On which medium are yeast plasmids transformed cells plated?

• a) Luria Broth

• b) Agar

• c) Minimal

• d) Nutrient

Plasmids are present in eukaryotes.

• a) True

• b) False

When YAC is used to clone DNA. What is the size of the DNA that can be cloned?

• a) Large (upto megabases)

• b) Small (upto few hundred bases)

• c) No size restriction

• d) Medium (upto kilobases)

TEL is the ______ sequence derived from the ends of ribosomal RNA-encoding molecules from the macromolecules of the protozoan Tetrahyena.

• a) centromeric

• b) telomeric

• c) can be either centromeric or telomeric

• d) can be present anywhere on the chromosome

Transformed cells in the case of YAC can be selected by the presence of both URA3 and TRP1 genes

• a) True

• b) False

Which of the following are advantageous to be used for expression of eukaryotic cells?

• a) Prokaryotic systems

• b) Yeast cells

• c) Fungi cells

• d) Algae cells

What are REP1 and REP2 genes in the plasmid used for?

• a) Origin

• b) Replication

• c) Expression

• d) Host lysis