PCR

What is the purpose of PCR?

• To produce millions of copies of DNA.
• To produce millions of copies of a specific region of DNA
• To add nucleotides to a DNA sequence
• To watch polymerase work.

At what temperature does the Denature step of PCR occur?

• 94-96° C
• 50-65° C
• 72° C
• Any temperature

Tags

NGSS.HS-PS1-2

NGSS.HS-PS1-5

Why does PCR require a specific type of polymerase?

• Taq polymerase can withstand the range of temperatures in PCR without denaturing.
• Taq polymerase is the most inexpensive type of polymerase.
• Taq polymerase is the most common type of polymerase.
• A specific type of polymerase is unnecessary.

The first step in PCR is

• Primer annealing

• Denaturation

• Primer extension

• None of these

What happens during the Anneal step of PCR?

• Primers are created.
• The primers attach to the target DNA region.
• Primers copy the new DNA strand.
• Primers sequence DNA

Does PCR require a primer?

• Yes

• No

What is the denaturation step?

• mutating RNA polymerase

• Cooling DNA to allow primers to form

• separating DNA helix in a cold environment

• separating DNA helix with using heat

What is the Extension step?

• DNA polymerase adds nucleotides to the 5' end

• RNA polymerase adds nucleotides to the 5' end

• DNA polymerase adds nucleotides to the 3' end

• RNA polymerase adds nucleotides to the 3' end

What process directly follows PCR?

• gel electrophoresis

• DNA sequencing

• cloning

• transformation

What is the purpose of the Extension step of PCR?

• To create the primers.
• To extend the time it takes to produce DNA.
• To allow polymerase to create the complementary strands of DNA.
• To allow substrates to copy DNA sequences.