PCR 13Q

What is the best definition for "primer"?

• An oligonucleotide of unknown sequence that is used to amplify the target gene.

• An oligonucleotide of known sequence that is used to amplify the target gene.

• An oligonucleotide of known sequence that is annealed to the target gene, allowing Taq polymerase to bind.

• A section of DNA on the target gene that is the binding site for Taq polymerase.

At what temperature does denaturation take place in PCR?

• 50 - 65^oC

• 94^oC

• 37^oC

• 72^oC

At what temperature does annealing of primers take place in PCR?

• 50 - 65^oC

• 94^oC

• 37^oC

• 72^oC

At what temperature does extension of the target gene take place in PCR?

• 50 - 65^oC

• 94^oC

• 37^oC

• 72^oC

What is the name of the bond that exists between bases of the coding and template strand of DNA? (Give the name, not the symbol)

(a)  

Why is denaturation the first step in PCR?

What is the name of the enzyme used to extend the target gene?

• Tac polymerase

• Taq polymerase

• DNA polymerase

• Yak polymerase

What is the advantage of using Taq polymerase in PCR as opposed to human DNA polymerase?

Name two lab procedures that rely on PCR.

What is the best definition for "dNTP"

• A nitrogenous base bound to a 5 carbon sugar with 3 phosphate groups bound to the sugar.

• A nitrogenous base bound to a 5 carbon sugar.

• The generic name for a nucleotide base.

• The generic name for a nucleoside base.