PCR 13Q

PCR 13Q

12th Grade - University

14 Qs

quiz-placeholder

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PCR 13Q

PCR 13Q

Assessment

Quiz

Biology

12th Grade - University

Easy

Created by

Mr A Lemin

Used 54+ times

FREE Resource

14 questions

Show all answers

1.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is the best definition for "primer"?

An oligonucleotide of unknown sequence that is used to amplify the target gene.

An oligonucleotide of known sequence that is used to amplify the target gene.

An oligonucleotide of known sequence that is annealed to the target gene, allowing Taq polymerase to bind.

A section of DNA on the target gene that is the binding site for Taq polymerase.

2.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

At what temperature does denaturation take place in PCR?

50 - 65oC

94oC

37oC

72oC

3.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

At what temperature does annealing of primers take place in PCR?

50 - 65oC

94oC

37oC

72oC

4.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

At what temperature does extension of the target gene take place in PCR?

50 - 65oC

94oC

37oC

72oC

5.

FILL IN THE BLANK QUESTION

1 min • 1 pt

What is the name of the bond that exists between bases of the coding and template strand of DNA? (Give the name, not the symbol)

6.

OPEN ENDED QUESTION

3 mins • 1 pt

Why is denaturation the first step in PCR?

Evaluate responses using AI:

OFF

7.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is the name of the enzyme used to extend the target gene?

Tac polymerase

Taq polymerase

DNA polymerase

Yak polymerase

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