What is the best definition for "primer"?
PCR 13Q

Quiz
•
Biology
•
12th Grade - University
•
Easy
Mr A Lemin
Used 54+ times
FREE Resource
14 questions
Show all answers
1.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
An oligonucleotide of unknown sequence that is used to amplify the target gene.
An oligonucleotide of known sequence that is used to amplify the target gene.
An oligonucleotide of known sequence that is annealed to the target gene, allowing Taq polymerase to bind.
A section of DNA on the target gene that is the binding site for Taq polymerase.
2.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
At what temperature does denaturation take place in PCR?
50 - 65oC
94oC
37oC
72oC
3.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
At what temperature does annealing of primers take place in PCR?
50 - 65oC
94oC
37oC
72oC
4.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
At what temperature does extension of the target gene take place in PCR?
50 - 65oC
94oC
37oC
72oC
5.
FILL IN THE BLANK QUESTION
1 min • 1 pt
What is the name of the bond that exists between bases of the coding and template strand of DNA? (Give the name, not the symbol)
6.
OPEN ENDED QUESTION
3 mins • 1 pt
Why is denaturation the first step in PCR?
Evaluate responses using AI:
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7.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
What is the name of the enzyme used to extend the target gene?
Tac polymerase
Taq polymerase
DNA polymerase
Yak polymerase
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