Sanger Sequencing

In the Sanger sequencing method, what causes the termination of each chain of DNA?

• The incorporation of a deoxynucleotide

• When the DNA polymerase encounters a stop codon

• The incorporation of a dideoxynucleotide

• Denaturation of the double strand

What is it called when the order of DNA nucleotides is determined for a section of DNA?

• DNA sequencing

• Denaturation

• Gel Electrophoresis

• DNA replication

Comparing DNA sequences between species can help us determine

• degree of similarity between species

• evolutionary relationships

• research potential pathogens

• all of the answers apply

Which of the following base pairs is matched correctly?

• Guanine and Thymine

• Cytosine and Adenine

• Adenine and Thymine

• Thymine and Cytosine

Which part of Sanger (Cycle) Sequencing is NOT found in regular Polymerase Chain Reaction (PCR)?

• DNA polymerase

• Primers

• Deoxyribonucleotides (dNTPs)

• Dideoxyribonucleotides (ddNTPs)

• DNA template

How are deoxyribonucleotides different from dideoxyribonucleotides? Choose all that apply.

• Deoxyribonucleotides are used in both PCR and Cycle Sequencing.

• Dideoxyribonucleotides are missing an OH group compared to deoxyribonucleotides.

• You cannot add new nucleotides to a deoxyribonucleotide.

• Deoxyribonucleotides are NOT found in natural living cells.

• Dideoxyribonucleotides are labeled with fluorescent tags.

How is DNA synthesis in PCR and Cycle Sequencing the SAME?

• Both require DNA polymerase.

• Both require primers.

• Both require deoxynucleotides (dNTPs).

• Both require dideoxynucleotides (dNTPs).

• Both generate new DNA products of equal length.

How is DNA synthesis in PCR and Cycle Sequencing DIFFERENT?

• Only PCR uses deoxynucleotides.

• Cycle Sequencing generates DNA products of different lengths.

• Only PCR analyzes DNA products using gel electrophoresis.

• Only Cycle Sequencing uses dideoxynucleotides.

What do dideoxynucleotides do when added to a growing DNA strand during Cycle Sequencing?

• They stop the elongation of that strand.

• That double the length of that strand.

• They shorten the length of the strand.

• They denature the strand.

Place the steps of DNA Sequencing below into their correct order.

A. Detection of fluorescence w/ lasers & sensors

B. PCR w/ fluorescent dideoxynucleotides

C. Separate DNA by size w/ gel electrophoresis

D. Match DNA sequence using BLAST database

• A, B, C, D

• B, C, A, D

• B, A, C, D

• D, B, C, A