ELISA Quizizz

Direct ELISA assay uses:

• a single antibody to recognize an antigen.

• two antibodies to recognize an antigen.

• uses starch indicator to recognize an antigen.

• PBS to recognize an antigen.

The colorimetric agent in the direct ELISA changes color from ________ to ___________.

• clear to blue

• clear to red

• blue to orange

• clear to green

Which enzyme causes the indicator TMB to change color in this lab?

• horseradish peroxidase

• lactase

• amylase

• bacillus subtillus

Tags

NGSS.HS-PS1-5

Which of the following is the main purpose of ELISA testing?

• to recognize an antigen.

• to identify antibodies.

• to detect COVID.

• to identify a protein and its concentration.

What was the first steps to the this lab?

• Add antibody .

• Add buffers to all the wells.

• Make a known standard set via serial dilution.

• Make antibody and antigen samples.

ELISA stands for

• Enzyme linked immune system absorbance

• Enzyme linked immunosorbent assay

• Enzyme linked ink assay

• Enzyme lined immediate assay

What is used to denature the enzyme, HRP, in this assay?

• acid

• base

• PBS

• antibody

At what temperature are these samples incubated?

• 32 degrees

• in the fridge

• in the freezer

• room temperature

Which solution is the acid in this assay?

• PBS

• Stop solution

• Colorimetric solution

• Antibody solution

What step will we be on for Thursday?

• 6

• 7

• 8

• 9