PAGE Gel Electrophoresis

Which of the following steps of PAGE electrophoresis would come FIRST?

• Pipette protein samples into wells

• Remove cassette from gel

• Immerse gel into stain

• Place casette in gel box at the correct orientation

SDS- PAGE Gel Electrophoresis is used to determine the molecular weight of ____.

Which of the following is a way that a PAGE gel is different than an agarose gel?

• PAGE gels are made of polyacrylamide

• PAGE gels often have a gradient of gel concentrations

• PAGE gels require the use of TBE buffer

• PAGE requires the use of SDS buffer

What is the function of SDS buffer in vertical electrophoresis?

• to track the passage of the sample

• it will ensure the proteins are denatured

• a reducing agent

• to increase the density of the sample

When placing a PAGE gel cassette in a gel box, the tall side of the cassette should be oriented towards...

• The side of the box with the gel cassette clip

• The middle of the gel box

Before protein samples can be loaded into wells, what needs to happen first?

• All buffer must be removed from wells

• The gel box needs to be turned on

• The protein samples need to be denatured in the water bath

• The protein samples need to be placed in an ice bath for 30 minutes

Proteins that are in their normal biologically active form are called...

Where can you find the positive electrode in a vertical gel electrophoresis box?

• At the top

• At the bottom

• On the side

Which of the following is NOT true of the gels used in PAGE electrophoresis?

• They have a concentration of 0.8%

• They allow the smallest proteins to migrate the farthest

• They must be purchased premade

• They are very thin and are therefore stored in a plastic cassette

In order to accurately estimate protein molecular weight using PAGE, the proteins must be...

• Isoelectric

• Native

• Denatured

• Positive