Light microscopy

The resolution limit of an optical microscope is approximately

• 25 nm

• 250 nm

• 2.5 μm

• 2.5 nm

The maximum resolution in an optical microscope is limited by…

• Diffraction

• Refraction

• Aberrations

• Equipment

Nanoscale x-ray imaging can be challenging due to…

• X-rays are hard to focus

• X-ray refractive index is 1 for most materials

• Accurate x-ray generation requires large national facilities

• All of the above

Super resolution imaging can surpass the diffraction limit

• True

• False

DNA sequencing requires nanoscale resolution images

• True

• False