BTC Downstrean 2

What rotor is best for withdrawing supernatant without disturbing the pellet?

• Swinging bucket

• Fixed angle

• Vertical

• None of the answers are correct.

In anion exchange chromatography,

• the column contains both positive and negatively charged beads where proteins bind depending on their net charge

• all of these

• the column contains positively charged beads where negatively charged proteins bind

• the column contains negatively charged beads where positively charged proteins bind

Which of the following is not a characteristic of preparative chromatography?

• Larger amounts of sample material

• Determine presence and relative proportion of analyte.

• Usually, large column diameter

• Isolate and purify analyte.

Which statement is correct?

• Preparative centrifugation is used to study pure compounds.

• Analytical centrifugation uses largre sample sizes

• Analytical centrifugation is used to study pure compounds.

• Preparative centrifugation uses small samples (<1ml)

With regards to Ion Exchange chromatography: At a buffer pH below its pI,

• a protein is positively charged and will bind to an anion exchanger.

• a protein is negatively charged and will bind to an anion exchanger.

• a protein is positively charged and will bind to a cation exchanger.

• a protein is negatively charged and will bind to a cation exchanger.

Thin layer chromatography is

• electrical mobility of ionic species

• adsorption chromatography

• all of these

• partition chromatography

What type of chromatography uses a liquid stationary phase?

• Partition

• Adsorption

• Affinity

• Ion-exchange

Define the analyte?

• It's the substance to be added during chromatography

• It's the substance to be mixed during chromatography

• It's the substance to be separated during chromatography.

• It's the substance to be identified during chromatography

 In which type of chromatography, the stationary phase held in a narrow tube and the mobile phase is forced through it under pressure?

• Both column and planar chromatography

• Planar chromatography

• Column chromatography

• Thin layer Chromatography

In a hydrophobic interaction chromatography if concentration of salt in the mobile phase is increased then

• amount of protein bound to the stationary phase increases.

• amount of protein bound to the stationary phase remains the same.

• amount of protein bound to the stationary phase decreases.

• salt has no effect on the protein.