High-Throughput Real-time PCR Workshop: Theory and Hands-On

1. Nucleic Acids (NA) are naturally occurring macromolecules that are necessary for life to exist.

• True

• False

Manual nucleic acid extraction is...

• Extremely fast

• Effortless

• Labor intensive

How many samples can the Maxwell RSC 16 process simultaneously?

• 24

• 2

• 16

• 8

The Maxwell® RSC Instruments work with a variety of sample types, including

human blood, cells and tissues.

• False

• True

The Maxwell® RSC Instruments use prefilled cartridges and preprogrammed

methods to reduce the potential for user error

• No

• Yes

By using a qPCR machine, qPCR reaction is visualized in real-time by recording fluorescence signal of dyes and probes at every PCR system

• False

• True

What is not the general features of conventional PCR?

• Using simple, non-labelled primers

• End-point detection with agarose gel electrophoresis

• Results obtained in Cq values

• Limited detection sensitivity

Azure Cielo™ Real-Time PCR system is designed to perform multiplex reaction, this will save time, cost, and sample.

• True

• False

qPCR provides greater detection sensitivity, therefore it is best for quantification

• True

• False

The following results can be obtained from quantitative Real-Time PCR (qPCR), except for:

• Melt curve

• Amplification curve

• Cq values

• Gel image