Ex 12B Creatinine FS

Concentration of the standard used

• 50mg/dL

• 0.2mmol/L

• 2mg/dL

• 5mg/dL

Composition of reagent 1

• NaOH

• Picric Acid

• Ba(OH)2

• H2SO4

TRUE OR FALSE: NaOH increases both the rate of formation of the colored complex and accelerates its breakdown

• TRUE

• FALSE

Wavelength used in this method

• 340nm

• 510nm

• 480nm

• 492nm

Blank used

• Reagent blank

• Air blank

• Water blank

Total reaction time

• 5mins

• 8mins

• 3mins

• 60 secs

Limit of detection

• 14mg/dL

• 0.2mg/dL

• 30mg/dL

• 1mg/dL

Conversion factor for Creatinine (mg/dL to mmol/L)

Composition of reagent 2

Optimum temperature

• Room temp

• 37C

• Boiling temp/100C

• 48C