Protoplast fusion

Plant protoplasts were used for ...........

• Breeding studies

• Morphology studies

• Cell studies

• Mutation studies

The process for making somatic hybrids are ........ exclude ?

• Cell culture

• Isolation

• Fusion

• PCR

(a)   explant samples were used for callus induction

which one is Jasminum spp. ?

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• 
• 
• 

Which enzyme was use in step of protoplast isolation ?

• Macerozyme

• Amylase

• DNase

• Endonuclease

What is optimal time of protoplast isolation ?

• 4 hr 26 oC

• 4 hr 37 oC

• 10 min 26 oC

• 10 min 37 oC

How long does it take for microcalli formation ?

• 6 weeks

• 8 weeks

• 10 weeks

• 12 weeks

How many percent of PEG was use in this study ?

• 10 %

• 20 %

• 30 %

• 40 %

How many protoplast cells for the best result of Protoplast transformation ?

• 40,000

• 400,000

• 4,000,000

• 40,000,000

Which one is correct for Protoplast ?

• cell without cell membrane

• hybrid cell

• only found in prokaryote

• can reproduce in stress condition