DNA Revision

What term best describes the arrangement of the two DNA strands?

What happens during the denaturation stage of PCR?

• Heat to 95oC and bonds break

• Heat to 95oC and hydrogen bonds break

• Heat to 95oC and hydrogen bonds between antiparallel strands break

• Heat to 95oC and the hydrogen bonds between the complementary antiparallel strands break

During PCR, where do the primers anneal during the annealing stage?

• Between 45-60 oC the RNA primers anneal to the complementary sequence

• Between 45-60 oC the DNA primers anneal to the complementary sequence

• At 72 oC the DNA primers anneal to the complementary sequence

• At 72 oC the DNA primers anneal to the bases on the leading strand

During elongation, which direction to the new nucleotides add to the growing strand?

• 5'-->3'

• 3'-->5'

How is DNA replicated in the cell?

• The leading strand replicates discontinuously and the lagging strand replicates continuously using RNA primers

• The leading strand replicates continuously and the lagging strand replicates discontinuously using DNA primers

• The leading strand replicates discontinuously and the lagging strand replicates continuously using DNA primers

• The leading strand replicates continuously and the lagging strand replicates discontinuously using RNA primers

The role of the ? is to bind to the operator

• inducer

• repressor

• lactose

The inducer binds to the repressor protein and....

• binds to the promoter and initiates transcription

• causes a conformational change to inhibit the repressor binding to the operator

• causes a conformational change to inhibit transcription

• inhibits polymerase

Introns are...

• Coding

• Non coding

What charge do histone proteins have?

• Positive

• Negative

Methylation of histone proteins is a change to the epigenome, what enzyme is inhibited?

• DNA polymerase

• Primase

• Transcription factor

• RNA polymerase