Sterilization and Aseptic Technique

What level of biosafety is appropriate for secondary education (7-12) laboratories?

• BSL-1

• BSL-2

• BSL-3

• BSL-4

When should you wash your hands with soap and water?

• After touching microorganisms

• Before and after handling materials

• Before handing materials

• When you get home from lab

How should microorganism cultures never be transferred?

• Plastic pipettes

• Using your fingers

• Inoculating Loops

• Sterile Swabs

Personal protective equipment would not include:

• Face mask

• Gloves

• Lab Coat

• Contact Lenses

Sterilization is most difficult on what type of bacteria?

• All types

• Spore-forming

• Gram-positive

• Gram-negative

Which of the following could best be used to sterilize metal or glassware?

• Running water

• Clorox wipe

• Autoclave or pressure cooker

• Hand soap

Which liquid could decontaminate petri dishes after culturing bacteria?

• Distilled water

• Vinegar

• Bleach

• Tap water

How often should you incinerate an inoculating loop during a bacterial culture transfer?

• After the transfer

• Before, during and after the transfer

• Before and after the transfer

• Before the transfer

Which of the following should be on or in a petri dish before incubation? Mark all that apply.

• Written label of name, species and date

• Nutrient agar or other growth medium

• Bacterial specimen

• Your lab notes

Which of the following could be used to sterilize liquid cultures? Check all that apply.

• Boiling

• Filtration

• Antibiotics

• Refridgeration