Lab 10 Biochemical tests intro

When bacteria are capable of fermentation, they are breaking down

• proteins

• lipids

• nucleic acids

• carbohydrates

Tags

NGSS.HS-LS1-6

If bacteria cannot ferment the sugar in fermentation media, what do they use to get ATP?

• fats in the media

• protein in the media

• they won't grow

• photosynthesis

Tags

NGSS.HS-LS2-3

When fermentation tubes contain phenol red (as we observed in lab), they appear what color when the pH is basic?

• yellow

• red

• green

• colorless

When fermentation tubes contain phenol red (as we observed in lab), they appear what color when the pH is ACIDIC?

• yellow

• red

• green

• colorless

If bacteria ferment the sugar in fermentation media with phenol red, then the media will appear

• red

• yellow

• colorless

• purple

If bacteria DO NOT ferment the sugar in fermentation media with phenol red, then the media will appear

• red

• yellow

• colorless

• purple

Selective media

• enriches for the growth of fastidious bacteria

• stops the growth of some types of microorganisms

• visually indicates if an enzyme is produced

• allows bacteria to grow only in selective temperatures

If you don't see bacterial growth in a fermentation tube, that indicates

• the bacteria fermented the sugar

• the bacteria did not ferment the sugar

• the results are inconclusive- need to repeat

• the pH indicator killed the bacteria

A Durham tube inside a fermentation tube

• captures gas that is produced

• allows bacteria to escape the acid

• allows the color change to happen

• captures all acid that is produced

Which is not an example of a rapid multi-biochemical test system? (choose all that apply)

• API Strip

• Enterotube

• serial dilutions

• Durham tube

• Vitek card