LCMS FST

Which of the following best describes the purpose of mass spectrometry?

• To measure the concentration of compounds in a sample.

• To generate charged molecules or molecular fragments and measure their masses.

• To separate different compounds in a mixture.

• To separate different compounds in a mixture.

3. Which component of an LCMS system is responsible for separating ions based on their mass-to-charge ratio?

• Column

• Detector

• Ionization Source

• Mass analyzer

Why is ionization a crucial step in mass spectrometry?

• To generate charged ions for easier manipulation with electric and magnetic fields.

• To reduce the mass of the compounds for improved resolution.

• To increase the volatility of the compounds.

• To stabilize the compounds for better analysis.

Which of the following is NOT a common ionization technique used in mass spectrometry?

• Electrospray ionization (ESI)

• Matrix-assisted laser desorption/ionization (MALDI)

• Gas chromatography (GC)

• Atmospheric pressure photoionization (APPI)

Which ionization technique utilizes a high-voltage potential applied to a fine liquid spray to generate ions?

• Electron impact ionization (EI)

• Matrix-assisted laser desorption/ionization (MALDI)

• Atmospheric pressure chemical ionization (APCI)

• Electrospray ionization (ESI)

Which of the following is NOT a type of mass analyzer used in mass spectrometry?

• Quadrupole

• Time-of-flight (TOF)

• Fourier transform ion cyclotron resonance (FT-ICR)

• High-performance liquid chromatography (HPLC)

In a TOF mass analyzer, how do lighter ions compare to heavier ions in terms of reaching the detector?

• Heavier ions reach the detector faster than lighter ions

• Lighter ions reach the detector faster than heavier ions

• Lighter and heavier ions reach the detector at the same time

• The flight times of ions are not dependent on their mass

What is the primary purpose of tandem MS/MS?

• Enhanced compound identification

• Determining molecular weight

• Analyzing isotopic abundance

• Measuring retention time

What type of information can be obtained through tandem MS/MS?

• Chromatographic peak area

• Elemental composition

• Retention time

• Molecular structural information

Which technique involves monitoring predefined precursor-to-product ion transitions to target specific analytes?

• Precursor ion scan

• Product ion scan

• Neutral loss scan

• Multiple reaction monitoring (MRM)