Cell Culture - Dr. Tosoc

It refers to the isolation of cells from an animal or plant and their subsequent growth in a favorable artificial environment.

• Plant Extraction

• Polymerase Chain Reaction

• Cell Culture

• DNA Extraction

This is derived directly from the primary culture. It has a uniform genotype and phenotype.

• Cell Strain

• Mature Cells

• Differentiated Cells

• Cell line

The cellular process by which they lose the ability to proliferate or divide.

• Discontinuation

• Senescence

• Termination

• Cessation

The type of cell culture where in the cells are free-floating in the culture medium.

• Suspension Cell Culture

• Hovered Cell Culture

• Adherent Cell Culture

• Attached Cell Culture

It is the percentage, which refers to the proportion of the culture dish or flask covered by adherent cells.

• Confluence

• Colonies

• Monolayer

• Cell Growth

Name the coating used for the cell culture flask specific for adherent cells.

• Polytetrafluoroethylene (PTFE)

• Silicon dioxide (SiO2) particles

• FluoroGlass® coating

• Poly-d-lysine (PDL) and collagen I coat

What is NOT a part of the criteria when choosing a cell line?

• Viral Risks

• Species

• Stability

• Availability

What is the Biosafety Level for a Cell Culture Laboratory?

• Biosafety Level 1

• Biosafety Level 2

• Biosafety Level 3

• Biosafety Level 4

What is the meaning of ATCC? It is one of the most reputable sources of cell lines.

• Academy of Test Culture Collection

• American Type Culture Collection

• Association of Tissue Culture Collection

• Authenticated Tissue Cell Culture

What is the specific enzyme used to detach adherent cells from the culture flask?

• Trypsin

• Translocase

• Transferase

• Hydrolase