DNA replication Prokaryotes and Eukaryotes

Chloramphenicol works by ____

• Increasing protein synthesis

• Halting protein synthesis

• Increasing DNA replication

• Halting DNA replication

In what phase of the cell cycle does DNA replication take place?

• G1
• S
• G2
• M

If a DNA strand contains the sequence 5'-TTAGGGACCACCTATA-3', which of the following is the sequence of the complementary strand of DNA?

• 3'-AATCCCTGGTGGATAT-5'

• 5'-AATCCCTGGTGGATAT-3'

• 3'-TTAGGGACCACCTATA-5'

• 5'-TTAGGGACCACCTATA-3'

Match the following genes involved in prokaryotic DNA replication with their function:

• Helicase loading protein

• dnaA

• Helicase

• dnaC

• Polymerase III alpha

• dnaE

• Initiator protein

• dnaG

• Primase

• dnaB

Mark the DNA binding domain of the initator protein.

DnaB helicase recruits the enzyme DnaG primase forming a complex called __________

Label the following diagram:

a

b

c

d

Gamma complex

Beta clamp

Psi and Chi proteins

Core polymerase

During replication, meeting of converging replication forks is known as ______.

The termination zone contains 10 ter sites (A–J), which can bind to ___ protein to form potent and polar replication fork barriers.

• Tis

• Tus

• Ter

• Tar

These polymerases have 3' - 5' exonuclease activity: ​​ (a)   ​ ​ ​ (b)  

• delta
• epsilon
• alpha
• beta