Microscopy 22/66

This microscope is commonly used for observing the surface details of specimens.

• Transmission Electron microscope (TEM)

• Compound light microscope

• Confocal microscope

• Scanning tunneling microscope (STM)

The purpose of staining specimens in light microscopy is to:

• Increase specimen size

• Add color for aesthetic appeal

• Improve contrast and visibility

• Make specimens conductive

The refractive index of a vacuum is:

• 0

• 1

• 10

• 100

Scanning Electron Microscopes (SEM) are primarily used for:

• Viewing live cells

• Examining surface topography

• Visualizing internal cellular structures

• Analyzing crystal structures

What distinguishes confocal microscopy from traditional fluorescence microscopy?

• It uses a laser to excite fluorophores.

• It has higher magnification capabilities.

• It can only visualize 2D samples.

• It lacks fluorescence capability.

The purpose of a wet mount in microscopy is to:

• Preserve specimens for long-term storage

• Enhance contrast in the specimen

• Observe living or delicate specimens in their natural state

• Prepare specimens for staining

When preparing a dry mount, what is typically used to adhere the specimen to the slide?

• Water

• Mounting medium

• Immersion oil

• A coverslip