QQ -4

What defines a palindromic sequence in DNA?

• Sequences with no symmetry

• Sequences that read the same forward and backward

• Sequences with overhanging ends

• Sequences with blunt ends

What type of bond does DNA ligase catalyze the formation of?

• Hydrogen bonds

• Peptide bonds

• Phosphodiester bonds

• Glycosidic bonds

How do plasmids replicate in relation to the bacterial chromosome?

• They replicate in conjunction with the bacterial chromosome.

• They rely on the bacterial chromosome for replication.

• They replicate independently of the bacterial chromosome.

• They do not undergo replication.

What is the function of the polylinker in PBR322 or pUC18/19?

• It encodes a protein for DNA replication.

• It contains multiple unique restriction enzyme recognition sites.

• It serves as a selectable marker for plasmid-containing cells.

• It facilitates transcription of genes.

What is the purpose of LacZ α-complementation in bacterial plasmids like pUC18?

• To synthesize glucose  

• To create blue-colored colonies

• To allow for blue-white screening of transformed colonies

• To promote DNA replication

When bacterial colonies contain the recombinant pUC18 plasmid with a disrupted lacZ gene, what color will they produce on X-gal plates?

• Blue

• Green

• Red

• White