Revision HPLC method development and validation

Which of the following parameters is NOT typically optimized during HPLC method development?

• Column temperature

• Detector wavelength

• Mobile phase pH
  

• Injection volume

• Magnification of the microscope

In the context of HPLC validation, which of the following best describes 'selectivity'?

• The ability of an analytical method to differentiate and quantify the analyte in the presence of other components.

• The consistency and reliability of test results when an analysis is repeated by different analysts, instruments, or labs.

• The degree of agreement between the true value and the value found.

• The smallest concentration of analyte in a sample which can be reliably detected but not necessarily quantitated.

• The ability of an analytical method to measure accurately and consistently the amount of analyte in various sample matrices.

During HPLC method validation, precision can be assessed at different levels. Which of the following is NOT a level of precision?

• Repeatability

• Intermediate precision

• Mobile phase precision

• Reproducibility

• Instrumental precision

Which of the following is an essential step in HPLC method development for ensuring peak resolution and short run times?

• Adjusting the flow rate of the mobile phase to the highest possible value.

• Choosing the right type of column.

• Using a single solvent as the mobile phase.

• Increasing the injection volume for better peak detection.

• Always using a UV detector.

Which term best describes the ability of a validated HPLC method to remain unaffected by small changes in method parameters and still produce consistent results?

• Linearity

• Specificity

• Accuracy

• Precision

• Robustness