BIO201- Ch13 Review

True or False: 

Using a simple diagnostic test is sufficient to identify a human pathogen in a clinical sample.

• True

• False

Which of the following is NOT a phenotype characterization useful for organism identification?

• Biochemical test

• Antigen test

• Cell culture

• Microscopy and staining

Acurate diagnosis of an infection disease requires...

• A physical exam noting all signs and symptoms

• Patient history

• Lab tests

• All of these answer are correct

An antibody is produced in response to...

• An agglutination

• PCR

• Antigen

• Enzyme

Why is gram staining NOT a definitive method to identify a pathogen?

• It will only identify a few pathogens

• There are multiple pathogenic species that will gram stain

• A gram stain cannot be used on blood cultures

• A gram stain is unreliable 37% of the time

PCR is used to...

• Test antibody sensitivity

• Sequence the genetic code of a pathogen

• Culture an organism that cannot be cultured by standard means

• Amplify DNA

To differentiate lactose and non-lactose fermentors, the medium used is...

• Triple sugar iron agar

• MacConkey agar

• Blood agar

• Chocolate agar

Based on your observation of this Gram stained specimen, select an appropriate statements.

• The pictured bacteria produce lipopolysaccharide (LPS)

• The pictured bacteria are Gram positive

• The pictured bacteria have a cell wall made up of mycolic acid

• The pictured bacteria are most likely acid-fast

• The pictured bacteria have teichoic acid embedded in its cell wall

Which of the following can be concluded from the following streaked MacConkey plate regarding sample A? (SELECT 2)

• Gram negative organism

• Gram positive organism

• They are able to ferment lactose

• They are unable to ferment lactose

The primary advantage to identifying pathogens using DNA analysis is that:

• DNA gives all the information about a pathogen.

• a pathogen does not have to be cultured.

• DNA analysis is the cheapest means of identification.

• all pathogens have been genetically sequenced and results are available in a matter of hours.