Aseptic Technique Quiz

What is the recommended disinfectant for cleaning the bench surface?

• Bleach

• Virkon solution

• Ethanol

• Hydrogen peroxide

When making transfers, what should be done if the bench surface is difficult to clean?

• Use a different bench

• Cover the bench with a sheet of tough material

• Leave the surface as it is

• Use a disinfectant spray

When should the operations be started?

• When the apparatus and materials are within immediate reach

• After taking a break

• After cleaning the work area

• After checking the weather forecast

What should be done while vessels are open?

• Work close to a heat source

• Work close to an open window

• Work close to a Bunsen burner flame

• Work close to a fan

How should the wire loop be sterilized before and after use?

• Wash it with soap and water

• Dip it in disinfectant solution

• Heat to red hot in a Bunsen burner flame

• Spray it with a sterilizing spray

What is the purpose of flaming the neck of bottles and test tubes?

• To cool down the vessel

• To create a convection current away from the opening

• To create a convection current towards the opening

• To add a pleasant aroma to the vessel

What is the recommended disinfectant for students to use for surface disinfection?

• Virkon solution

• Bleach

• Ethanol

• Hydrogen peroxide

How should the wire loop be held while sterilizing it in a Bunsen burner flame?

• At a 45-degree angle

• Upside down

• Vertically

• Horizontally

What should be done after flaming the neck of a bottle or test tube?

• Put down the cap/cotton wool plug

• Wave the vessel around

• Replace the cap/cotton wool plug on the vessel

• Leave the vessel open

How long should the surface be left wet after disinfection with 1% Virkon solution?

• 15 minutes

• 5 minutes

• 20 minutes

• 10 minutes