biochem spotter

Flow cytometry

• a procedure that analyses physical and chemical properties of each cell in a mixed population of cells

• lab machine used for separating particles in a medium based on density and size

• Different reagents are used for separating DNA, or RNA or proteins from a tissue sample

• separate biomolecules (DNA or RNA or protein or hemoglobin) based on their size, shape and charge.

Centrifugation

• a procedure that analyses physical and chemical properties of each cell in a mixed population of cells

• lab machine used for separating particles in a medium based on density and size

• Different reagents are used for separating DNA, or RNA or proteins from a tissue sample

• separate biomolecules (DNA or RNA or protein or hemoglobin) based on their size, shape and charge.

Extraction of Biomolecules

• a procedure that analyses physical and chemical properties of each cell in a mixed population of cells

• lab machine used for separating particles in a medium based on density and size

• Different reagents are used for separating DNA, or RNA or proteins from a tissue sample.

• separate biomolecules (DNA or RNA or protein or hemoglobin) based on their size, shape and charge.

electrophoresis

• Quantitative analysis is done by converting the substance into a colored substance which can absorb light.

• separate biomolecules (DNA or RNA or protein or hemoglobin) based on their size, shape and charge.

• It is a physical method used for separating components in a mixture by their ability to move through different phases of materials.

• Different reagents are used for separating DNA, or RNA or proteins from a tissue sample.

Chromatography

• Cprocedure that analyses physical and chemical properties of each cell in a mixed population of cells

• It is a physical method used for separating components in a mixture by their ability to move through different phases of materials.

• separate biomolecules (DNA or RNA or protein or hemoglobin) based on their size, shape and charge.

• Quantitative analysis is done by converting the substance into a colored substance which can absorb light.

Spectrophotometer

• Quantitative analysis is done by converting the substance into a colored substance which can absorb light.

• lab machine used for separating particles in a medium based on density and size

• Flow cytometry is a procedure that analyses physical and chemical properties of each cell in a mixed population of cells

• Different reagents are used for separating DNA, or RNA or proteins from a tissue sample.

Uses of flow cytometry

• Cell counting

  Cell sorting

  Biomarker detection

  Diagnosis of health disorders such as leukemia

• Separation of organelles

  Purification of samples

• Amplification of desired sequence of DNA

• Millions of copies of desired sequence of DNA can be ,made in few hours.

Uses Centrifugation

• Separation of organelles

  Purification of samples

• Purification of mixture of biomolecules

• Biomarker detection

• Amplification of desired sequence of DNA

Uses electrophoresis

• To separate a mixture of biomolecules.

• Millions of copies of desired sequence of DNA can be ,made in few hours.

• FPurification of mixture of biomolecules:

• Amplification of desired sequence of DNA

Pcr

• Amplification of desired sequence of DNA

  Millions of copies of desired sequence of DNA can be ,made in few hours.

• It is a physical method used for separating components in a mixture by their ability to move through different phases of materials.

• Purification of mixture of biomolecules: