MI4.1.2 Modules 2&3 Pre-Lab

Which plate from Module 1 will you be using to make your seed culture?

• -DNA

• -DNA/+Amp

• +DNA/+Amp

• +DNA/+Amp/+IPTG

How many colonies will you pick with the loop to make your seed culture?

• all of them

• 1-2

• 3-4

• 4-5

• as many as you can see

Do not allow the plates to incubate longer than ____ hours at 37C.

Mathematical Equivalence

ON

Tags

NGSS.HS-PS3-4

How will you be able to tell which plate has the most GFP expression after incubation?

• brightness of the glow

• number of colonies

• if the colonies are a continuous lawn

• if the colonies are individual dots

• by weighing the plate

________ means spinning a tube at high speed to separate components based on density.

_____ is the process of breaking open a cell to release its contents.

Cells with lysis buffer will freeze faster if you position the tube on its

Tags

NGSS.MS-PS1-4

NGSS.MS-PS3-4

Which of the following is NOT a process meant to help lyse the cells?

• hot water bath

• lysis buffer

• freezing

• vortexing

What does it mean if the pellet is fluorescent instead of the supernatant?

• the cells are still intact

• the cells have lysed

• the experiment has failed

• the proteins have denatured

To tell if the supernatant is glowing you will probably need to

• expose it to UV light

• shake it alot

• go into a dark room

• speak kindly to it

• live, laugh, love

Tags

NGSS.MS-PS4-2