Recombinant DNA technology

Define recombinant DNA technology

• A method in which same DNA from same organisms is spliced (combine) together in the laboratory

• A method in which DNA is spliced together in the laboratory

• A method in which different DNA from same organism is spliced (combine) together in the laboratory

• A method in which DNA from different organisms is combined together in the laboratory

What are the typical characteristics of a cloning vector?

• Bacterial cells cannot survive without it when grown under certain conditions.
• It contains restriction sites that allow the insertion of foreign DNA segments.
• It can replicate in bacterial cells.
• All of the above.

 Which of the following statement about plasmid is correct?
I ) It can replicate by itself
II ) Hybrid plasmid is introduced into bacteria by transformation
III ) Other than bacteria, plasmid can also be found in bacteriophage

• I only
• I and II only
• II and III only
• I,II and III

What enzyme forms covalent bonds between restriction fragments?

• DNA primase
• DNA helicase
• DNA ligase
• DNA polymerase

Which of the following is NOT a tool used in recombinant DNA technology?

• Cloning vector

• RNA polymerase

• Target DNA

• DNA ligase

A cloning vector is

• An agent that transfers a pathogen

• An agent that transfers a foreign gene from one DNA molecule to another

• An agent that transfers a DNA segment to a RNA molecule

• An agent that transfers a RNA segment to a DNA molecule

Which of the following statements describes a modifying enzyme?

• It cuts the DNA to obtain the gene of interest

• It breaks the DNA molecule to produce multiple fragments

• It stimulates the replication of a recombinant DNA

• It seals the inserted gene of interest to an open plasmid

Which of the following is TRUE regarding screening by using X-gal?

• Antibiotics will prevent the growth of bacteria without plasmid

• Bacteria cells with recombinant plasmids are resistant to antibiotics and are unable to hydrolyse X-gal

• Bacteria cells which contain non-recombinant plasmids are resistant to antibiotics and unable to hydrolyse X-gal

5 tools used in recombinant DNA technology

• Target DNA, Taq polymerase, DNA cloning vector, host cell, modifying enzymes

• Target DNA, restriction enzyme, DNA cloning vector, host cell, modifying enzyme

• Target gene, restriction enzymes, DNA cloning vector, host cell, DNA polymerase

• Target gene, restriction enzyme, DNA cloning vector, host cell, cDNA

Which of the following is CORRECT about host cell?

• Plasmid is an example of host cell.

• Unable to receive DNA molecule through transformation.

• An organism that prevents cloning vectors from multiplying.

• An organism that is used to receive recombinant DNA for cloning purposes.