HPLC instrument

Student ID

Which one is not the characteristics of HPLC that is different from that of traditional LC?

• particle size

• column size

• separation efficiency

• separation mechanism

From the parts listed below, please put a correct order to show a block diagram of HPLC.

1. 1. column, 2. detector, 3. pump, 4. injector, 5. MP

• 3, 5, 4, 1, 2

• 5, 3, 4, 1, 2

• 5, 3, 1, 4, 2

• 4, 5, 3, 1, 2

Which detector is different from others?

• Refractive index

• UV-Vis absorbance

• Fluorescence

Which detector provides the highest sensitivity?

• Refractive index

• UV-Vis spectroscopy

• Fluorescence

Which pump is used for low pressure gradient mixing of MP?

• quaterary pump

• binary pump

• ternary pump

• monopoly pump

Methanol (MeOH) has a UV cut-off at 205 nm. Which wavelength should be used for UV detection if MeOH is used as an MP ?

• 190 nm

• 200 nm

• 205 nm

• 220 nm

Which of the following is not the properties of a guad column?

• use the same SP as the analytical column

• a short column

• positioned before the analytical column

• used for preconcentration of analytes

which one is correct for "Load" position of 6-port valve?

• sample fills the loop and goes to waste

• sample in the loop goes to a column

• MP goes to the loop

• MP goes to waste

what is gradient elution?

• the composition of MP is changed during one run.

• The flow rate of MP is changed during one run.

• The temperature of the column is changed during one run.

• The composition of MP is fixed during one run.