Protein Analysis

Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis

Single-Stranded DNA Polymerase Gel Electrophoresis

Serum-Derived Stem-Platelet Aggregation Electrophoresis

Standard Differential Staining-Photoelectric Gel Electrophoresis

Breaks disulphide bonds

Imparts a negative charge

It denatures proteins

Enhances protein movement through the gel

Provides structural support for proteins

To separate proteins by size

To facilitate protein denaturation

To enhance protein staining

Charge

Size

Shape

Hydrophobicity

By the intensity of staining

By comparing the sample to known molecular weight markers

Calculations using the gel percentage, voltage, and time

Luck

To provide a platform for sample loading

To separate proteins based on size

To concentrate proteins in a narrow band so they enter the resolving gel at the same time

To enhance protein denaturation

To give the protein a net negative charge

To improve protein solubility

To reduce disulphide bonds

To improve protein transfer

Transfer

Electrophoresis

Blocking

Antibody Incubation

To amplify signal intensity

To aid antibody binding

To prevent nonspecific binding of antibodies

To aid in protein visualisation

To block nonspecific binding

To enhance signal intensity

To directly bind the target protein

To separate proteins by size