What is Polymerase Chain Reaction (PCR)? | miniPCR bio™

What does PCR stand for?

• Polymerase Chain Reaction

• Polymer Chain Replication

• Protein Chain Replication

• Protein Chain Reaction

What is the main purpose of PCR?

• To make copies of a specific DNA sequence

• To observe DNA under a microscope

• To change the structure of DNA

• To decrease the amount of DNA

Which base does Adenine (A) pair with in DNA?

• Adenine (A)

• Thymine (T)

• Guanine (G)

• Cytosine (C)

What is the first step of PCR?

• Replication

• Extension

• Denaturation

• Annealing

What happens to the DNA strands during the denaturation step of PCR?

• They are copied

• They are cut

• They are joined together

• They are separated

What is the role of primers in PCR?

• To join the DNA strands

• To cool down the reaction

• To specify the region of DNA to be copied

• To break the DNA strands

At what temperature does the extension step occur in PCR?

• 95°C

• 50-65°C

• 72°C

• 37°C

What enzyme is used in PCR to synthesize new DNA strands?

• Ligase

• Helicase

• Taq Polymerase

• Restriction Enzyme

Where was Taq Polymerase originally found?

• In the deep sea

• In thermophilic bacteria in hot springs

• In the human body

• In the Amazon rainforest

Why is Taq Polymerase used in PCR?

• Because it can replicate RNA

• Because it remains stable at high temperatures

• Because it is easily available

• Because it works at low temperatures