Forensics DNA Analysis

What is a DNA Fingerprint?

• A DNA Fingerprint is a method of identification that is able to determine the pattern of a person’s DNA.

• The carrier of genetic information.

• A real fingerprint.

Step one of making a DNA fingerprint

• Obtain the DNA

• Extract and Isolate the DNA

• PCR (Polymerase Chain Reaction)

DNA must be kept cool, dry, and out of direct sunlight when being transported. Otherwise the evidence may be compromised or grow mold.

• PCR (Polymerase Chain Reaction)

• Obtain the DNA

• Extract and Isolate the DNA

Step two of making a DNA fingerprint

• PCR (Polymerase Chain Reaction)

• Obtain the DNA

• Extract and Isolate the DNA

Cells are first extracted from biological evidence and are then disrupted (centrifuged) in order to release the DNA from proteins and other cell components. Once released, DNA can be extracted from the nucleus of the cell.

• PCR (Polymerase Chain Reaction)

• Obtain the DNA

• Extract and Isolate the DNA

Step three of making a DNA fingerprint

• Extract and Isolate the DNA

• PCR (Polymerase Chain Reaction)

• Use Restriction Enzymes

Known for using small, trace amounts of DNA and generating multiple identical copies.

• PCR (Polymerase Chain Reaction)

• Use Restriction Enzymes

• Gel Electrophoresis

Step four of making a DNA fingerprint

• PCR (Polymerase Chain Reaction)

• Use Restriction Enzymes

• Gel Electrophoresis

The DNA is then mixed with ________, which cut the DNA in specific places that will result in different sized fragments.

• Gel Electrophoresis

• PCR (Polymerase Chain Reaction)

• Restriction Enzymes

Step five of making a DNA fingerprint

• Gel Electrophoresis

• Use Restriction Enzymes

• Analyze DNA