CRISPR-Cas9 Basics

What does CRISPR stand for?

• Clustered Regularly Interspaced Short Patterns

• Crispy Regularly Interspaced Short Palindromes

• Clustered Regularly Inserted Short Repeats

• Clustered Regularly Interspaced Short Palindromic Repeats

Tags

NGSS.HS-LS3-2

What is the function of Cas9 in the CRISPR system?

• Cuts DNA at specific locations

• Regulates gene expression

• Binds to ATP

• Transcribes RNA

Which organism originally had the CRISPR-Cas9 system?

• Plants

• Fungi

• Virus

• Bacteria

Explain the process of CRISPR-Cas9 gene editing.

• The process of CRISPR-Cas9 gene editing involves using a guide RNA to target a specific DNA sequence, which is then cut by the Cas9 enzyme. The cell's natural DNA repair mechanisms can introduce desired changes during the repair process.

• The Cas9 enzyme introduces changes directly to the DNA sequence

• The repair process in CRISPR-Cas9 gene editing is not influenced by the cell's natural mechanisms

• CRISPR-Cas9 gene editing involves using a guide DNA instead of RNA

Tags

NGSS.HS-LS3-2

What are the potential applications of CRISPR-Cas9 technology?

• Application in space exploration

• Gene editing for medical purposes, agriculture, and research

• CRISPR-Cas9 for cooking recipes

• Gene editing for entertainment purposes

Tags

NGSS.HS-LS2-7

What is the significance of the PAM sequence in CRISPR-Cas9?

• The PAM sequence is only found in bacteria.

• The PAM sequence is responsible for cutting the target DNA.

• The PAM sequence is necessary for Cas9 to bind to the target DNA.

• The PAM sequence is not essential for CRISPR-Cas9 function.

How does CRISPR-Cas9 differ from other gene editing techniques?

• CRISPR-Cas9 is less efficient than other gene editing techniques.

• CRISPR-Cas9 is more precise and efficient than other gene editing techniques.

• CRISPR-Cas9 is more error-prone than other gene editing techniques.

• CRISPR-Cas9 is slower than other gene editing techniques.

What are the ethical concerns surrounding the use of CRISPR-Cas9?

• Use of CRISPR-Cas9 in agriculture

• Lack of public awareness

• Genetic modification of humans, unintended consequences, unequal access, regulatory oversight

• Potential for misuse by governments

Tags

NGSS.HS-ETS1-3

What are the limitations of CRISPR-Cas9 technology?

• Incompatibility with certain cell types

• Off-target effects, unintended mutations, delivery challenges, ethical concerns

• Lack of specificity

• Regulatory hurdles

Tags

NGSS.HS-ETS1-3

How has CRISPR-Cas9 impacted the field of biotechnology?

• CRISPR-Cas9 has led to a decrease in the accuracy of gene editing

• CRISPR-Cas9 has increased the cost of biotechnological research

• CRISPR-Cas9 has no impact on biotechnology

• CRISPR-Cas9 has revolutionized biotechnology by providing a precise and efficient tool for gene editing.