MUMGP Open Day Quiz

1. 1. Whole genome sequencing refers to:

• Sequencing of coding portions of genes in a genome.

• Sequencing of complete mitochondrial DNA.

• Sequencing of chloroplast DNA.

• None of the above.

2. 2. Current sequencing technologies are capable of sequencing entire human chromosomes without any gaps.

• True.

• False.

3. 3. DNA Sequencing can identify the following types of human genetic variations?

• Simple tandem repeats.

• Indels.

• Single nucleotide polymorphisms.

• All of the above

4. 4. Illumina sequencing is a short-read sequencing platform.

• True.

• False.

5. 5. Human whole genome sequencing using Illumina, Oxford Nanopore Technologies or PacBio sequencing include ALL, except the following step, in generating the contiguous genome sequences.

• DNA extraction from biological tissues or environments.

• Library preparation.

• Use of DNA polymerase enzyme.

• Massively parallel sequencing.

6. 6. The human reference genome (GRCh38) contains approximately this number of protein coding genes.

• 10,000

• 20,000

• 50,000

• 100,000

7. 7. Which of the following are long-read sequencers?

• PacBio Onso, Element Biosciences AVITI

• ONT PromethION 2, Illumina MiSeq

• PacBio Sequel IIe, ONT MinION

• PacBio Sequel IIe, MGI DNBSEQ

8. 8. Which instrument is recommended by PacBio to access the size of high molecular weight DNA?

• Agilent TapeStation 4200

• Agilent Femto Pulse

• Agilent Bioanalyzer

• Thermo Fisher NanoDrop 2000

9. 9. Which of the following service is not provided by MUMGP?

• Bacterial whole genome sequencing

• Variant calling analysis

• 16S rRNA amplicon sequencing

• Single-cell sequencing

10. 10. What is the operating system used at the MUMGP Bioinformatics and Big Data Laboratory?

• Linux

• macOS

• Microsoft Windows

• ChromeOS