Cloning Quiz

What is the role of plasmids in the process of cloning?

• They are used to cut DNA at specific sites.

• They are circular DNA molecules that replicate independently of the rest of the bacterial DNA.

• They are proteins that help in the synthesis of DNA.

• They are enzymes that break down bacterial cell walls.

What can cloning be used for?

• To create new species of bacteria.

• To reproduce DNA molecules or mass-produce proteins.

• To destroy harmful bacteria in the environment.

• To create new types of viruses.

Which of the following is NOT a step in the cloning process as described in the diagram?

• Inserting the gene of interest into a plasmid.

• Putting the plasmid into a bacterial cell.

• Growing host cells in culture to form a clone.

• Using the cloned cells to create new animal species.

What is the primary function of restriction enzymes?

What type of ends do restriction enzymes create when they cut DNA?

What is one application of restriction enzymes in genetic engineering?

Which of the following statements is true about the cuts made by restriction enzymes?

What is a cDNA library?

• A) A collection of DNA sequences from the entire genome

• B) A collection of DNA sequences for the genes in the cell that are being sequenced

• C) A collection of RNA sequences from the cell

• D) A collection of protein sequences from the cell

How is ssDNA constructed in the cell?

• A) Using DNA polymerase

• B) Using RNA polymerase

• C) Using reverse transcriptase from mRNA

• D) Using ribosomes

What enzyme is responsible for adding a complementary DNA strand in the construction of cDNA?

• A) DNA ligase

• B) DNA polymerase

• C) RNA polymerase

• D) Helicase