Microscopy Measurement Questions

An eyepiece graticule is primarily used to:

• Illuminate the specimen

• Measure the size of objects viewed under the microscope

• Focus the image

• Increase the magnification

Explain how to calibrate an eyepiece graticule using a stage micrometer. Include the steps involved in the process.

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If a specimen measures 15 divisions on the eyepiece graticule and each division corresponds to 2 micrometers on the stage micrometer, what is the actual size of the specimen in micrometers?

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The stage micrometer is a tool used to view specimens, while the eyepiece graticule is a fixed measurement scale used for calibration.

• True

• False

Describe the significance of using the correct units (e.g., micrometers, millimeters) when reporting measurements taken with an eyepiece graticule and stage micrometer.

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Match the following terms with their correct descriptions:

• Eyepiece Graticule

• Stage Micrometer

• Micrometer

• Calibration

The eyepiece graticule is often calibrated against a stage micrometer to ensure accurate _______________ in measurements. The units typically used for measuring cells are ____________ and ____________.

• Measurements

• micrometers

• millimeters