Exploring Body Cells Under the Microscope

In this video, the presenter demonstrates how to observe cheek cells under a microscope using different imaging techniques: bright field, differential interference contrast (DIC), and phase contrast. The video also covers the process of staining cells with methylene blue to enhance visibility. The presenter provides a detailed walkthrough of the materials needed, sample preparation, and the step-by-step process of using each microscopy technique. The video concludes with a discussion on the differences between the techniques and an invitation to subscribe to the channel for more microscopy content.

What is the purpose of using methylene blue in this experiment?

• To adjust the light intensity

• To increase the magnification

• To stain the cheek cells

• To clean the microscope

Which material is used to collect cheek cell samples?

• Cover glass

• Microscope slide

• Pipette

• Q-tips

What is the first imaging technique used to observe the cheek cells?

• Bright field

• Phase contrast

• DIC

• Dark field

Why is it important to remove excess water from the slide?

• To prevent the objectives from getting wet

• To increase the number of cells

• To enhance the color of the stain

• To improve the light intensity

What does DIC stand for?

• Digital Interference Contrast

• Dynamic Imaging Contrast

• Direct Illumination Contrast

• Differential Interference Contrast

What is the visual effect of using DIC on the cells?

• It makes the cells appear flat

• It gives a three-dimensional appearance

• It darkens the background

• It removes the nuclei

What is a notable artifact seen in phase contrast microscopy?

• Blurred edges

• Darkened nuclei

• Brighter halo around structures

• Increased cell size

What happens when phase contrast is combined with DIC?

• An interesting overlap occurs

• The cells disappear

• The image becomes blurry

• The light intensity decreases

Why does the presenter add methylene blue after placing the slide on the microscope?

• To clean the slide

• To increase the number of cells

• To prevent the cells from drying out

• To show how the stain spreads and is absorbed by the cells

What is the effect of methylene blue on the cells?

• It makes the cells larger

• It dissolves the cells

• It stains the cells blue

• It makes the cells transparent