Quiz on Isolation and Extraction of Nucleic Acids

What is the primary purpose of isolating DNA?

• To convert DNA into RNA

• To destroy the DNA

• To increase the size of the DNA

• To analyze or manipulate DNA

Which method is NOT mentioned as a way to disrupt cell membranes?

• Mechanical disruption

• Chemical lysis

• Enzymatic digestion

• Thermal denaturation

What is the role of EDTA in the lysis buffer?

• To provide osmotic balance

• To chelate Mg2+ and block nucleases

• To solubilize membrane proteins

• To maintain pH

Which of the following is a common source for DNA isolation?

• Light

• Water

• Blood

• Air

What is the purpose of using ice-cold ethanol in DNA precipitation?

• To dissolve DNA

• To degrade DNA

• To enhance DNA solubility

• To precipitate DNA

Which type of RNA is primarily involved in protein synthesis?

• miRNA

• mRNA

• rRNA

• tRNA

What is a significant challenge in RNA extraction compared to DNA extraction?

• RNA extraction is simpler than DNA extraction

• RNA is less abundant in cells

• RNA is more susceptible to degradation by RNases

• RNA is more stable than DNA

What is the function of proteinase K in DNA isolation?

• To stabilize RNA

• To precipitate DNA

• To digest proteins

• To lyse cells

Which method is used for plasmid DNA isolation?

• SDS-PAGE

• Alkaline lysis method

• Ethanol precipitation

• Phenol-chloroform extraction

What is the main reason for using a chaotropic agent in RNA extraction?

• To precipitate RNA

• To denature proteins

• To enhance RNA yield

• To stabilize RNA