TEST DBB30703

Name:

Matrix no.

In DNA extraction, DNA is precipitate using

• A. 70% ethanol.
• B. 50% phenol.
• C. 80% chloroform.
• D. 20% Tris EDTA buffer.

Isolation steps of DNA and RNA are

• A. Separating nucleic acid-opening cell-recovering nucleic acid.
• B. Opening cell-recovering nucleic acid-separating nucleic acid.
• C. Separating nucleic acid-recovering nucleic acid-opening cell.
• D. Opening cell-separating nucleic acid-recovering nucleic acid.

Homogenising of cell is done to

• A. seperate cells
• B. disolve cells
• C. break the cell wall
• D. seperate DNA

The Basic Local Alignment Search tool (BLAST) is used to

• A. rapid search of nucleotide in a database
• B. design fragment of a gene
• C. find similarity of a mutation
• D. determine the protein involved in a mutation

Annealing is a process of ___________ in PCR

• A. DNA extension
• B. primer binding to DNA
• C. visualisation of DNA segment
• D. breaking the DNA double strand

Restriction enzyme is used in determination of mutation is by

• A. cutting the normal nucleotide in a gene
• B. recognising the mutation at specific gene
• C. creating sticky end of the mutated nucleotide
• D. combining two or more bands after restriction process

Restriction enzyme cuts the DNA producing

• A. sticky end
• B. mirror end
• C. straight end
• D. inverted end

Enzyme that involve in DNA synthesis is called

• A. Ligase.
• B. Polymerase.
• C. Exonuclease.
• D. Endonuclease.