Exploring Biotechnology and Genetics

Which of the following gene editing techniques uses a guide RNA to target specific DNA sequences for editing?

• Zinc Finger Nucleases (ZFNs)

• CRISPR-Cas9

• TALENs

• RNA Interference (RNAi)

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DOK Level 2: Skill/Concept

Explain how Mendel's Law of Independent Assortment applies to the formation of gametes in a dihybrid cross.

• It states that alleles for different traits are inherited together.

• It states that alleles for different traits segregate independently during gamete formation.

• It states that alleles for the same trait segregate together.

• It states that alleles for the same trait are inherited independently.

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DOK Level 3: Strategic Thinking

Match the following phases of the cell cycle with their primary functions.

• Mitosis and cell division

• M phase

• Cell growth and preparation for DNA replication

• S phase

• Preparation for mitosis

• G2 phase

• DNA replication

• G1 phase

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DOK Level 2: Skill/Concept

Discuss the ethical considerations involved in genetic engineering, particularly in the context of human gene editing.

• It is universally accepted and poses no ethical dilemmas.

• It raises concerns about consent, potential long-term effects, and genetic inequality.

• It is only concerned with the technical feasibility of gene editing.

• It is primarily focused on the economic benefits of genetic engineering.

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DOK Level 3: Strategic Thinking

Which of the following is a common application of biotechnology in agriculture?

• Cloning of endangered species

• Development of genetically modified crops for pest resistance

• Use of CRISPR for human gene therapy

• Production of synthetic insulin

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DOK Level 2: Skill/Concept

Analyze the role of cyclins and cyclin-dependent kinases (CDKs) in cell cycle regulation.

• They are not involved in cell cycle regulation.

• They form complexes that regulate the progression of the cell cycle.

• They are only involved in DNA replication.

• They inhibit cell cycle progression.

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DOK Level 3: Strategic Thinking

What is the primary ethical concern associated with the use of CRISPR technology in human embryos?

• It is too expensive to use widely.

• It may lead to unintended genetic mutations.

• It is not effective in editing genes.

• It is difficult to administer.

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DOK Level 2: Skill/Concept

Describe the process of DNA replication, including the role of key enzymes.

• DNA replication is a simple process that does not involve enzymes.

• DNA replication involves the unwinding of the double helix and the synthesis of a new strand by DNA polymerase.

• DNA replication occurs without the need for a template strand.

• DNA replication is a process that only occurs in prokaryotes.

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DOK Level 3: Strategic Thinking

Which of the following best describes Mendel's Law of Segregation?

• Alleles for a trait are inherited together.

• Alleles for a trait separate during gamete formation.

• Alleles for different traits are inherited independently.

• Alleles for a trait do not separate during gamete formation.

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DOK Level 2: Skill/Concept

Evaluate the potential benefits and risks of using biotechnology in medicine.

• Biotechnology has no risks and only benefits.

• Biotechnology can lead to personalized medicine but also raises concerns about privacy and genetic discrimination.

• Biotechnology is only beneficial for agricultural applications.

• Biotechnology is too risky to be used in medicine.

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DOK Level 3: Strategic Thinking