Cell cloning and Contamination Quiz

What is commonly method used for suspension cell cloning?

• Matrigel

• Agar

• Agarose

• All of the given option

What is a potential source of viral contamination in cell culture?

• Cell lines from reputable sources

• Serum

• Cold stores

• CO2 Incubators

What is the purpose of using a cloning ring?

• To improve plating efficiency

• To calculate plating efficiency

• To isolate individual clones

• To increase number of clones

What is a NOT a sign of bacterial contamination in cell cultures?

• Increased pH

• Color change in the medium

• Cloudiness of medium

• Spots on growth surface

What is a NOT a method used to detect mycoplasma contamination in cell cultures?

• PCR testing

• Fluorescence staining

• Microbiological culture

• Colorimetric assay

Which of the following is NOT a possible cause of cross-contamination of cell lines?

• Sharing work space

• Not carrying out authentication checks

• Poor inventory control in freezer

• None of the given options

Calculate the number of clones that will form in a single microtitration well from a cell suspension of 100 cells/ml having a plating efficiency of 5%.

• 1

• 0

• 0-1

• 5

Why is Methocel preferred for suspension cell cloning?

• easier to handle

• easier to view clones

• does not solidify

• All of the given options

In case of a __________ contamination, one should discard all media, stock solutions, trypsin, etc. in current use

• recurring and multispecific

• non-widespread

• widespread

• multispecific

One can detect viral contamination in cell culture using the following EXCEPT?

• Phase-contrast microscopy

• Polymerase chain reaction

• ELISA

• Immunostaining