Enzyme Activity and Experimental Design

The video tutorial by Miss Esterick Biology covers how to plan and write up a required practical on enzymes, specifically focusing on the effects of pH on trypsin activity. It introduces Mathsify Tuition as a resource for students needing extra help. The tutorial details the aim, hypothesis, equipment, method, results, and analysis of the experiment. It also discusses graphing techniques, drawing conclusions, and evaluating the method's limitations.

What is the primary purpose of Mathsify Tuition as mentioned in the video?

• To organize group study sessions

• To provide free online courses

• To offer one-to-one tutoring for students needing extra help

• To sell educational materials

Which of the following is NOT a factor that could be investigated in the enzyme practical?

• Enzyme concentration

• Temperature

• Substrate concentration

• Light intensity

What is the substrate used in the experiment to test the effect of pH on trypsin?

• Gelatin

• Protein

• Starch

• Lactose

What is the purpose of using a buffer solution in the experiment?

• To maintain a constant temperature

• To keep the enzyme at a specific pH

• To increase the reaction speed

• To act as a substrate

How is the endpoint of the reaction determined in the experiment?

• When the pH reaches 7

• When the solution turns blue

• When the film becomes completely clear

• When the temperature reaches 50°C

What should be done if no change is observed in the film after 25 minutes?

• Increase the temperature

• Record the time as infinity

• Record the time as zero

• Continue the experiment indefinitely

Why is it important to identify anomalous results when calculating the mean?

• To improve the accuracy of the mean

• To reduce the number of trials needed

• To make the experiment faster

• To ensure the results are as high as possible

What is the main reason for converting the rate of reaction to times 10 to the minus three?

• To reduce the number of calculations

• To simplify the experiment

• To increase the reaction rate

• To make the data easier to plot

What conclusion can be drawn about trypsin's activity at pH 4?

• Trypsin is inactive at all pH levels

• Trypsin is most active at pH 4

• Trypsin works best at pH 4

• Trypsin is denatured at pH 4

Which of the following is a limitation of the method used in the experiment?

• The substrate is not suitable

• The buffer solution is too strong

• The endpoint is subjective

• The enzyme concentration is too high