Biotechnology HW 8.2

What is transformation in the context of recombinant DNA?

• The uptake and expression of foreign DNA by a cell

• The process of cell division

• The breakdown of DNA in a cell

• The replication of DNA within a cell

What was one of the first transformations involving human insulin?

• Inserting the human insulin gene into E. coli cells

• Transferring human insulin into mammalian cells

• Using human insulin to treat bacterial infections

• Creating synthetic insulin in a laboratory

What is transduction in the context of using viruses for transformation?

• The use of viruses to transform or genetically engineer cells

• The process of DNA replication in viruses

• The breakdown of viral DNA in host cells

• The use of bacteria to transform viral DNA

What is the role of plasmid DNA in genetic engineering?

• To carry genes of interest into cells

• To replicate DNA within the cell

• To break down foreign DNA

• To produce insulin in mammalian cells

What is a plasmid?

• A large, linear DNA molecule

• A small, circular, double-stranded DNA molecule

• A protein that cuts DNA

• A type of RNA

What is the role of restriction enzymes in DNA manipulation?

• To replicate DNA

• To splice RNA

• To cut DNA strands at specific sequences

• To synthesize proteins

What are "sticky ends" in DNA?

• Ends of DNA that are blunt and do not pair

• Ends of DNA that are longer on one side and can pair with complementary sequences

• Ends of DNA that are circular

• Ends of DNA that are linear

What is the role of restriction enzymes in bacteria?

• To replicate DNA

• To defend against invading viral DNA

• To synthesize proteins

• To repair DNA

What is a palindrome in the context of restriction enzyme sequences?

• A sequence that reads the same forward and backward

• A sequence that forms a loop

• A sequence that is identical to another sequence

• A sequence that is unique to each organism

What is the significance of restriction fragment length polymorphism (RFLP) analysis?

• It helps in DNA replication

• It reveals information about differences in DNA sequences

• It assists in protein synthesis

• It is used for RNA transcription

Tags

NGSS.HS-LS4-3