Restriction Enzymes and Gel Electrophoresis

Which suspect matches that of the crime scene DNA?

• 1

• 2

• 3

• 4

Tags

NGSS.HS-LS3-1

Which suspect matches the crime scene?

• Suspect 1

• Suspect 2

• Neither

Tags

NGSS.HS-LS3-2

Which male is the father of the child?

• Male 1

• Male 2

Tags

NGSS.HS-LS3-1

NGSS.HS-LS3-2

Which suspect's DNA fingerprint matches that of the felon?

• S1

• S2

• S3

• None

What cuts the DNA prior to running gel electrophoresis?

• DNA polymerase

• Helicase

• Ligase

• Restriction enzyme

What does a thicker band represent in a gel?

• There’s more DNA material present in that band

• There’s more glucose available in that band

• There’s more mutation in the DNA fragment present

• There’s more radioactive material present in that band

What is used to cut the DNA into fragments for the gel?

• Ligase from eukaryotes

• Ligase from prokaryotes

• Restriction enzymes from eukaryotes

• Restriction enzymes from prokaryotes

Which DNA tech separates fragments by size/charge?

• Gel electrophoresis

• PCR

• Sequencing

• Transformation

Minimum restriction sites for ECO on plasmid to get this?

• 1

• 2

• 3

• 4

Gel electrophoresis separates molecules based on

• Charge and Polarity

• Polarity and Weight

• Weight and Size

• Size and Charge