Gel Electrophoresis
Flashcard
•
Biology
•
9th - 12th Grade
•
Hard
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15 questions
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1.
FLASHCARD QUESTION
Front
What is a point mutation?
Back
A point mutation is a genetic mutation where one nucleotide base is changed, resulting in the alteration of a single amino acid in a protein.
2.
FLASHCARD QUESTION
Front
What is gel electrophoresis?
Back
Gel electrophoresis is a laboratory technique used to separate DNA, RNA, or proteins based on their size and charge.
3.
FLASHCARD QUESTION
Front
What are the base-pairing rules in DNA?
Back
The base-pairing rules in DNA are A-T (adenine pairs with thymine) and G-C (guanine pairs with cytosine).
4.
FLASHCARD QUESTION
Front
Why are enzymes added to DNA before electrophoresis?
Back
Enzymes are added to DNA to cut the DNA into fragments, which allows for separation during the electrophoresis process.
5.
FLASHCARD QUESTION
Front
What is the purpose of gel electrophoresis in genetic analysis?
Back
The purpose of gel electrophoresis in genetic analysis is to separate and visualize DNA fragments for purposes such as DNA fingerprinting, cloning, and sequencing.
6.
FLASHCARD QUESTION
Front
What is the role of the gel in gel electrophoresis?
Back
The gel acts as a medium through which DNA fragments can migrate; smaller fragments move faster and farther than larger ones.
7.
FLASHCARD QUESTION
Front
What is the significance of the electric field in gel electrophoresis?
Back
The electric field causes the negatively charged DNA fragments to migrate towards the positive electrode, facilitating their separation.
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