Protein Sequencing Quiz

Protein sequencing can be performed without breaking the protein into smaller fragments.

• True

• False

Answer explanation

Proteins must be cleaved into smaller fragments for sequencing.

Edman degradation cleaves proteins at the N-terminal.

• True

• False

Carboxypeptidase A cleaves the last residue at the C-terminal of a protein.

• True

• False

Acid hydrolysis cleaves all peptide bonds in a protein.

• True

• False

Mass spectrometry cannot be used for determining the molecular mass of peptides.

• True

• False

Answer explanation

1. Mass spectrometry is a primary tool for determining peptide molecular masses.

Mass spectrometry uses the mass-to-charge ratio (m/z) to identify molecules.

• True

• False

The overlap method constructs protein sequences by aligning overlapping peptides.

• True

• False

Edman degradation uses PITC (Phenylisothiocyanate) as a reagent.

• True

• False

Protein sequences stored in databases include details about disulfide bonds and post-translational modifications.

• True

• False

X-ray crystallography requires protein crystallization.

• True

• False