Gel Electrophoresis

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What is Gel Electrophoresis?

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A laboratory technique used to separate DNA fragments based on their size by applying an electric field to a gel matrix.

Front

What charge does DNA carry?

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DNA is negatively charged due to its phosphate backbone.

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What is the role of a Restriction Enzyme in DNA analysis?

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Restriction enzymes cut DNA at specific sequences, allowing for the analysis of DNA fragments.

Front

What is the purpose of using a gel in Gel Electrophoresis?

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The gel acts as a medium through which DNA fragments can migrate, allowing for separation based on size.

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How does the size of DNA fragments affect their movement in Gel Electrophoresis?

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Smaller DNA fragments move faster and travel further through the gel than larger fragments.

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What is the significance of the buffer solution in Gel Electrophoresis?

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The buffer solution maintains a stable pH and provides ions that conduct electricity during the electrophoresis process.

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What is the typical gel used in Gel Electrophoresis?

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Agarose gel is commonly used for separating DNA fragments.

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What is the purpose of loading dye in Gel Electrophoresis?

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Loading dye helps visualize the sample and allows for tracking the progress of electrophoresis.

Front

What is the function of a DNA ladder in Gel Electrophoresis?

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A DNA ladder serves as a molecular weight marker to estimate the size of DNA fragments in the sample.

Front

What happens to DNA fragments when an electric current is applied in Gel Electrophoresis?

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DNA fragments migrate towards the positive electrode due to their negative charge.