lecture 19

What are the three main steps of translation?

• Initiation, Elongation, Termination

• Replication, Transcription, Translation

• Initiation, Replication, Termination

• Transcription, Translation, Replication

Which of the following is NOT a part of the elongation step in translation?

• Decoding

• Peptide bond formation

• Translocation

• Replication

What is the first step in the initiation of translation in E. coli?

• Binding of the 50S subunit

• Binding of IF-1 and IF-3 to the 30S subunit

• Recruitment of the first tRNA

• Hydrolysis of GTP by IF-2

Which molecule is required to recruit the first tRNA during translation initiation?

• IF-1

• IF-3

• IF-2/GTP

• 50S subunit

What is the role of IF-1 and IF-3 during translation initiation?

• To hydrolyze GTP

• To recruit the first tRNA

• To keep subunits apart until ready and recruit mRNA

• To bind the 50S subunit

What is the activated form of the first tRNA used in translation initiation?

• Met-tRNA

• fMet-tRNA

• Ala-tRNA

• Gly-tRNA

In prokaryotes, what does the first tRNA contain instead of methionine?

• N-formyl methionine

• Just methionine

• N-acetyl methionine

• N-methyl methionine

In eukaryotes, what is the first tRNA?

• N-formyl methionine

• Just methionine

• N-acetyl methionine

• N-methyl methionine

What is the role of EF-Tu in the elongation cycle?

• It delivers aminoacyl tRNA to the ribosome.

• It catalyzes peptide bond formation.

• It facilitates translocation of the ribosome.

• It removes GDP from the ribosome.

During the elongation cycle, what happens after the peptidyl transfer?

• Translocation occurs.

• Decoding begins.

• EF-Tu binds to GTP.

• The ribosome disassembles.