Quiz on Nucleic Acid Detection and Electrophoresis

What is the primary purpose of electrophoresis?

• To amplify DNA

• To separate molecules based on size and charge

• To visualize nucleic acids

• To synthesize proteins

Which gel type is derived from seaweed?

• Agarose Gel

• Capillary Gel

• Silica Gel

• Polyacrylamide Gel

What is the role of buffer systems in electrophoresis?

• To enhance the visibility of the samples

• To increase the temperature of the gel

• To carry the current and protect samples

• To provide nutrients to the samples

Which dye is most widely used for DNA analysis?

• Ethidium Bromide

• Fluorescein

• Silver Stain

• SyBr Green

What is the typical concentration range for low-concentration agarose gels?

• 2000-50,000 bp

• 1-10%

• 50-500 bp

• 10-20%

What is the purpose of using pulsed field gel electrophoresis?

• To synthesize nucleic acids

• To visualize proteins

• To enhance migration of very large DNA pieces

• To separate small DNA fragments

Which of the following is a common buffer used in electrophoresis?

• Acetic acid

• Tris borate EDTA (TBE)

• Hydrochloric acid

• Sodium chloride

What is the main advantage of polyacrylamide gels?

• Higher resolution for small fragments

• Lower cost

• More durable

• Easier to prepare

What does a pure preparation of DNA typically have as an OD260/OD280 ratio?

• 1.0-1.5

• 2.5-3.0

• 1.8-2.0

• 0.5-1.0

What is the function of the comb in electrophoresis equipment?

• To heat the gel

• To stir the buffer solution

• To create wells for sample loading

• To measure DNA concentration