Restriction Enzymes and Gel Electrophoresis

In preparation for an electrophoresis procedure, enzymes are added to DNA in order to

• convert the DNA into gel

• cut the DNA into fragments

• change the color of the DNA

• produce longer sections of DNA

A student performed a gel electrophoresis experiment. The results are represented in the diagram

below. Compared to the fragments at the top of the gel, the fragments at the lower end are

• larger, and move slower

• larger, and move faster

• smaller, and move faster

• smaller, and move slower

Restriction Enzymes come from . . . 

• viruses
• bacteria
• goats
• spiders

What charge is DNA?

• positive
• negative
• neutral

Which two bear species are most closely related?

• Bear 1 and Bear 2
• Bear 1 and Bear 3
• Bear 2 and Bear 3
• There’s no way to tell

Tags

NGSS.MS-LS4-2

NGSS.MS-LS4-3

Why do the fragments of DNA in gel electrophoresis travel away from the negative electrode?

• DNA is negatively charged
• DNA is positively charged
• the agarose gel in negatively charged
• the agarose gel is positively charged

During gel electrophoresis, DNA moves from the ___ end to the ___ end.

• positive, negative
• negative, positive

Tags

NGSS.HS-PS2-5

When using restriction enzymes, what is used to glue together DNA fragments 

• ligases
• plasmids
• bacteriophages

In order to insert a human gene into plasmid, both must 

• code for the same gene product.
• be cut by the same restriction enzyme.
• originate from the same type of cell.
• have identical sequences.

Restriction Enzymes . . . 

• cut DNA at precise locations
• help bacteria to pick up DNA
• move DNA through a gel
• copy DNA